custom written matlab script matlab r2020a  (MathWorks Inc)

Journal: Frontiers in Physiology

Article Title: Calcium Signaling in T Cells Is Induced by Binding to Nickel-Chelating Lipids in Supported Lipid Bilayers

doi: 10.3389/fphys.2020.613367

Figure Lengend Snippet: T-cell signaling fraction increases with concentration of DGS-NTA(Ni) in the SLB. (A) Schematic illustration showing calcium signaling by either ( left ) DGS-NTA(Ni) in an SLB or ( right ) anti-CD3 antibodies on glass. (B) The fraction of signaling T cells for either ligand-free SLBs with DGS-NTA(Ni) or OKT3 on a glass slide (mean signaling fraction ± s.d.). (C) The time between cell landing and calcium signaling for either ligand-free SLBs with DGS-NTA(Ni) or OKT3 on a glass slide (mean signaling time ± s.d.). (D) The signaling fraction binding to a ligand-free SLB containing 5 wt% DGS-NTA(X), with X being either Co 2+ , Ni 2+ or Cu 2+ . (E) The signaling fraction of J.RT3-T3.5 cells ( left ) and Jurkat T cells ( right ) on SLBs with ligand-free 5 wt% DGS-NTA(Ni) and on OKT3-coated glass. Data are shown for three to five experiments, except for E which is from two experiments, with 150 cells in each experiment. A two-sample t -test in MATLAB was used for statistical analysis with: * <0.05, ** <0.01, *** <0.001.

Article Snippet: Calcium signaling from the bound cells was analyzed using a custom-written MATLAB script (R2020a, MathWorks).

Techniques: Concentration Assay, Binding Assay